Sphere-Formation Assay: Three-Dimensional in vitro Culturing of Prostate Cancer Stem/Progenitor Sphere-Forming Cells
نویسندگان
چکیده
منابع مشابه
Effect of Radiation on self-renewality of prostate cancer stem cells.
Introduction: CSCs have been identified in prostate cancer (PCa), one of the most diagnosed malignancies in men over the world, for which radiation resistance is a major problem in the treatment of advanced stages. Cancer stem cells (CSCs) have the ability to self-renew and differentiate to give rise to heterogeneous phenotype of the tumor cells. It is believed that CSCs are ...
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PURPOSE To isolate precursors of human corneal endothelial cells (HCECs) in vitro. METHODS HCECs were subjected to a sphere-forming assay in which spheres floated in serum-free medium containing growth factors. To promote differentiation, the isolated sphere colonies were plated in dishes coated with poly-L-lysine (PLL)/laminin or fetal bovine endothelium extracellular matrix. Marker expressi...
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The order-disorder transition of a sphere-forming block copolymer thin film was numerically studied through a Cahn-Hilliard model. Simulations show that the fundamental mechanisms of pattern formation are spinodal decomposition and nucleation and growth. The range of validity of each relaxation process is controlled by the spinodal and order-disorder temperatures. The initial stages of spinodal...
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We perform Monte Carlo simulations of the hard-sphere lattice gas on the body-centred cubic lattice with nearest neighbour exclusion. We get the critical exponents, β/ν = 0.311(8) and γ/ν = 2.38(2), where β, γ, and ν are the critical exponents of the staggered density, the staggered compressibility, and the correlation length, respectively. The values of the hard-sphere lattice gas on the simpl...
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BACKGROUND Resistance to radiotherapy continues to be a limiting factor in the treatment of cancer including head and neck squamous cell carcinoma (HNSCC). Simultaneous targeting of β1 integrin and EGFR was shown to have a higher radiosensitizing potential than mono-targeting in the majority of tested HNSCC cancer models. As tumor-initiating cells (TIC) are thought to play a key role for therap...
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ژورنال
عنوان ژورنال: Frontiers in Oncology
سال: 2018
ISSN: 2234-943X
DOI: 10.3389/fonc.2018.00347